A TURBIDIMETRIC METHOD FOR THE ASSAY OF HYALURONIDASE
نویسندگان
چکیده
منابع مشابه
A turbidimetric method for the assay of hyaluronidase.
A variety of methods have been devised for the assay of the enzyme hyaluronidase. These may be divided into four types: (a) measurement of the “spreading effect” in the skin of rabbits and guinea pigs (1, 2), (b) reduction of viscosity (3,4), (c) prevention of mucin clot formation (5), and (d) chemical measurement of total reducing substances, N-acetylglucosamine, or glucuronic acid liberated o...
متن کاملCorrelation of reductimetric and turbidimetric methods for hyaluronidase assay.
When hyaluronidases act on isolated (as distinct from native) sodium hyaluronate, three changes in the properties of the substrate solution can be measured: (1) the decrease in viscosity, (2) the loss of ability to form insoluble protein salts on acidification, (3) the increase in reducing sugar content.’ Each of these changes is the basis for a quantitative method of measurement of enzyme acti...
متن کاملThe turbidimetric estimation of hyaluronidase.
2. Tests with known mixtures of phosphate esters and the recovery of the phosphorus of muscle extracts from the chromatograms suggest that the individual values obtained with the method in its present form are reliable to within 10 %. 3. Trichloroacetic acid extracts of muscle have been found to contain certain phosphate esters not yet identified. 4. The pyrophosphate fraction present in the ex...
متن کاملTurbidimetric method for the assay of antiviral antibodies.
A rapid, simple assay method has been developed for antiviral antibodies. The technique has been applied to antisera, immune gamma-globulins, and immunospecifically purified antibody for two strains of influenza virus, Asian 305 and PR8, and to antisera to tobacco mosaic virus. Turbidity changes due to the specific interaction of a virus with its antibody were measured by the increase in optica...
متن کاملinvestigating the feasibility of a proposed model for geometric design of deployable arch structures
deployable scissor type structures are composed of the so-called scissor-like elements (sles), which are connected to each other at an intermediate point through a pivotal connection and allow them to be folded into a compact bundle for storage or transport. several sles are connected to each other in order to form units with regular polygonal plan views. the sides and radii of the polygons are...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1948
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)52721-1